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dc.contributor.authorAhmed W.H.-
dc.contributor.authorBoulet, Nathalie-
dc.contributor.authorBriot, Anaïs-
dc.contributor.authorRyan B.J.-
dc.contributor.authorKinsella, Gemma K.-
dc.contributor.authorO’sullivan, Jeffrey-
dc.contributor.authorLes Parellada, Francisco-
dc.contributor.authorMercader-Barceló, Josep-
dc.contributor.authorHenehan, Gary T. M.-
dc.contributor.authorCarpéné, Christian-
dc.date.accessioned2021-09-17T14:07:19Z-
dc.date.available2021-09-17T14:07:19Z-
dc.date.issued2021-06-23-
dc.identifier.citationAhmed, W.H.; Boulet, N.; Briot, A.; Ryan, B.J.; Kinsella, G.K.; O’Sullivan, J.; Les, F.; Mercader-Barceló, J.; Henehan, G.T.M.; Carpéné, C. Novel Facet of an Old Dietary Molecule? Direct Influence of Caffeine on Glucose and Biogenic Amine Handling by Human Adipocytes. Molecules 2021, 26, 3831. https://doi.org/10.3390/molecules26133831es_ES
dc.identifier.issn1420-3049es_ES
dc.identifier.urihttps://repositorio.usj.es/handle/123456789/605-
dc.description.abstractCaffeine is a plant alkaloid present in food and beverages consumed worldwide. It has high lipid solubility with recognized actions in the central nervous system and in peripheral tissues, notably the adipose depots. However, the literature is scant regarding caffeine’s influence on adipocyte functions other than lipolysis, such as glucose incorporation into lipids (lipogenesis) and amine oxidation. The objective of this study was to explore the direct effects of caffeine and of isobutylmethylxanthine (IBMX) on these adipocyte functions. Glucose transport into fat cells freshly isolated from mice, rats, or humans was monitored by determining [3H]-2-deoxyglucose (2-DG) uptake, while the incorporation of radiolabeled glucose into cell lipids was used as an index of lipogenic activity. Oxidation of benzylamine by primary amine oxidase (PrAO) was inhibited by increasing doses of caffeine in human adipose tissue preparations with an inhibition constant (Ki) in the millimolar range. Caffeine inhibited basal and insulin-stimulated glucose transport as well as lipogenesis in rodent adipose cells. The antilipogenic action of caffeine was also observed in adipocytes from mice genetically invalidated for PrAO activity, indicating that PrAO activity was not required for lipogenesis inhibition. These caffeine inhibitory properties were extended to human adipocytes: relative to basal 2-DG uptake, set at 1.0 ± 0.2 for 6 individuals, 0.1 mM caffeine tended to reduce uptake to 0.83 ± 0.08. Insulin increased uptake by 3.86 ± 1.11 fold when tested alone at 100 nM, and by 3.21 ± 0.80 when combined with caffeine. Our results reinforce the recommendation of caffeine’s potential in the treatment or prevention of obesity complications.es_ES
dc.format.extent18es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPI AGes_ES
dc.relation.requiresAdobe PDFes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCaffeinees_ES
dc.subjectAdipocytees_ES
dc.subjectLipolysises_ES
dc.subjectLipogenesises_ES
dc.subjectAmine oxidaseses_ES
dc.subjectMethylxanthineses_ES
dc.subjectGlucose transportes_ES
dc.titleNovel facet of an old dietary molecule? Direct influence of caffeine on glucose and biogenic amine handling by human adipocyteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/26/13/3831es_ES
dc.identifier.publicationfirstpage1es_ES
dc.identifier.publicationlastpage18es_ES
dc.identifier.doi10.3390/molecules26133831es_ES
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses_ES
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