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dc.contributor.authorCasedas, Guillermo-
dc.contributor.authorMaggi, Filippo-
dc.contributor.authorMazzara, Eugenia-
dc.contributor.authorLópez Ramos, Víctor-
dc.contributor.authorMoliner Langa, Ana Cristina-
dc.date.accessioned2023-01-31T09:40:24Z-
dc.date.available2023-01-31T09:40:24Z-
dc.date.issued2022-09-13-
dc.identifier.issn1663-9812en_US
dc.identifier.urihttps://repositorio.usj.es/handle/123456789/892-
dc.descriptionCannabis sativa L. is a plant that contains numerous chemically active compounds including cannabinoids such as trans-Δ-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD), and flavone derivatives, such as luteolin-7-Oglucuronide and apigenin glucuronide. In particular, the polar fraction of hemp including many phenolic compounds has been overlooked when compared with the more lipophilic fraction containing cannabinoids. Therefore, the aim of this study was to assess two extracts of industrial hemp (C. sativa) of different polarity (aqueous and hexane) by evaluating their antioxidant profile and their neuroprotective potential on pharmacological targets in the central nervous system (CNS). Several assays on in vitro antioxidant capacity (DPPH, superoxide radical, FRAP, ORAC), as well as inhibition of physiological enzymes such as acetylcholinesterase (AChE) and monoaminooxidase A (MAO-A) were carried out in order to find out how these extracts may be helpful to prevent neurodegenerative disorders. Neuro-2a cell line was selected to test the cytotoxic and neuroprotective potential of these extracts. Both extracts showed striking antioxidant capacity in the FRAP and ORAC assays, particularly the hexane extract, and interesting results for the DPPH and superoxide radical uptake assays, with the aqueous extract standing out especially in the latter. In enzyme inhibition assays, the aqueous extract showed AChE and MAO-A inhibitory activity, while the hexane extract only reached IC50 value for AChE inhibitory bioassay. Neuro-2a assays demonstrated that polyphenolic extract was not cytotoxic and exhibited cytoprotective properties against hydrogen peroxide and antioxidant response decreasing reactive oxygen species (ROS) production. These extracts could be a source of compounds with potential benefit on human health, especially related to neurodegenerative disorders.en_US
dc.format.extent11 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoengen_US
dc.publisherSelcuk University, Science Facultyen_US
dc.relation.requiresadobeen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjecttrans-Δ-9-tetrahydrocannabinol (Δ9-THC)en_US
dc.subjectcannabidiol (CBD)en_US
dc.subjectcannabinoiden_US
dc.subjectpolyphenolen_US
dc.subjectoxidative stressen_US
dc.subjectantioxidanten_US
dc.subjectenzymatic inhibitionen_US
dc.subjectcentral nervous system (CNS)en_US
dc.titleEvaluation of two different Cannabis sativa L. extracts as antioxidant and neuroprotective agentsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.identifier.doi10.3389/fphar.2022.1009868en_US
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessen_US
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