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dc.contributor.authorRoig, Francisco J.-
dc.contributor.authorAmaro Gonzalez, Carmen-
dc.contributor.authorAlcaine Otín, Alejandro-
dc.contributor.authorCarro, Jesús-
dc.identifier.citationRoig Molina FJ, Amaro González C, Alcaine Otín A and Carro Fernández J (2023) Vibrio vulnificus mutation rate: an in vitro approach. Front. Microbiol. 14:1223293. doi: 10.3389/fmicb.2023.1223293en_US
dc.description.abstractVibrio vulnificus is a multi-host pathogenic species currently subdivided into five phylogenetic lineages (L) plus one pathovar with the ability to infect fish due to a transmissible virulence plasmid. This plasmid (or a fragment of it) has been transmitted between lineages within the species, contributing to the evolution of V. vulnificus. This study aimed to provide an experimental approximation to the V. vulnificusmutation rate by determining spontaneousmutation rates frombacterial cultures of representants of the di􀀀erent lineages by whole-genome sequencing. To this purpose, synonymous SNP di􀀀erences, i.e., spontaneous mutation not subjected to the evolutive forces, between initial and final culture after serial growth were evaluated and used for mutation rate calculationen_US
dc.format.extent9 p.en_US
dc.relationThis study was partially funded by Departamento de Ciencia, Universidad y Sociedad del Conocimiento, from the Gobierno de Aragon (Spain) (Research Group T71_23D). This study also forms part of the ThinkInAzul program and was supported by grant THINKINAZUL/2021/027 from MCIN (Ministerio de Ciencia e Innovacion de Espana) with funding from European Union NextGeneration EU (PRTR-C17.I1) and GV (Generalitat Valenciana). The study was also supported by grants PID2020-120619RB-I00 funded by MCIN/AEI/10.13039/501100011033 and AICO/2020/076 funded by GV. This study was also supported by Universidad San Jorge (internal call for Open Access publication grants 2022-2023).en_US
dc.rightsAtribución 4.0 Internacional*
dc.subjectMutation rateen_US
dc.subjectNumber of generationsen_US
dc.subjectVibrio vulnificusen_US
dc.titleVibrio vulnificus mutation rate: an in vitro approachen_US
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