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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Valero Gracia, Marta Sofía | - |
dc.contributor.author | López Ramos, Víctor | - |
dc.contributor.author | Castro, Marta | - |
dc.contributor.author | Gómez-Rincón, Carlota | - |
dc.contributor.author | Arruebo, María Pilar | - |
dc.contributor.author | Les Parellada, Francisco | - |
dc.contributor.author | Plaza, Miguel Ángel | - |
dc.date.accessioned | 2023-10-24T11:26:40Z | - |
dc.date.available | 2023-10-24T11:26:40Z | - |
dc.date.issued | 2022-11-20 | - |
dc.identifier.citation | Valero, M.S.; López, V.; Castro, M.; Gómez-Rincón, C.; Arruebo, M.P.; Les, F.; Plaza, M.Á. Involvement of NO/cGMP Signaling Pathway, Ca2+ and K+ Channels on Spasmolytic Effect of Everlasting Flower Polyphenolic Extract (Helichrysum stoechas (L.) Moench). Int. J. Mol. Sci. 2022, 23, 14422. https://doi.org/10.3390/ ijms232214422 | en_US |
dc.identifier.issn | 1422-0067 | en_US |
dc.identifier.uri | https://repositorio.usj.es/handle/123456789/1008 | - |
dc.description.abstract | Functional gastrointestinal diseases (FGID) are worldwide prevalent conditions. Pharmacological treatments can be ineffective, leading the population to turn to herbal or traditional remedies. Helichrysum stoechas (L.) Moench is a medicinal plant traditionally used in the Iberian Peninsula to treat digestive disorders, but its effects on gastrointestinal motility have not been scientifically demonstrated. The aim of this work was to evaluate the antispasmodic effect of a polyphenolic extract of H. stoechas (HSM), its mechanism of action and its antioxidant activity. Isometric myography studies were performed in rat ileum, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) levels were measured in rat jejunum. HSM reduced the integrated mechanical activity of spontaneous contractions. In Ca2+-free medium, HSM reduced the concentration–response curve of CaCl2 similarly to verapamil. Pre-incubation with the extract blocked the contraction induced by Bay K8644, KCl and carbachol. L-NAME, ODQ, Rp-8-Br-PET-cGMPS, KT-5823, apamin, TRAM-34 and charybdotoxin reduced the relaxant effect of the extract on spontaneous contractions. MDA+4-HDA levels in LPS-treated tissue were reduced by the extract, showing antioxidant activity. In conclusion, HSM showed antispasmodic activity through inhibition of Ca2+ influx, activation of the NO/PKG/cGMP pathway and opening of Ca2+-activated K+ channels. The results suggest that H. stoechas could help in the prevention or treatment of FGIDs. | en_US |
dc.format.extent | 17 p. | en_US |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.relation | This research was funded by Government of Aragon (financial support of the group B44_20D-Phyto-Pharm: Principios vegetales bioactivos y ciencias farmaceuticas), Universidad de Zaragoza (JIUZ-2018-BIO-09 and JIUZ-2021-BIO-08) and Universidad San Jorge (Internal Research Project Call 2021-2022, number 2122041). | en_US |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Antioxidant | en_US |
dc.subject | Antispasmodic activity | en_US |
dc.subject | Ion channels | en_US |
dc.subject | Gastrointestinal diseases | en_US |
dc.subject | Medicinal plants | en_US |
dc.subject | Polyphenols | en_US |
dc.title | Involvement of NO/cGMP Signaling Pathway, Ca2+ and K+ Channels on Spasmolytic Effect of Everlasting Flower Polyphenolic Extract (Helichrysum stoechas (L.) Moench) | en_US |
dc.type | info:eu-repo/semantics/article | en_US |
dc.identifier.doi | https://doi.org/10.3390/ ijms232214422 | en_US |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | en_US |
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