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dc.contributor.authorCásedas, Guillermo-
dc.contributor.authorLes, Francisco-
dc.contributor.authorLópez Ramos, Víctor-
dc.contributor.authorChoya-Foces, Carmen-
dc.contributor.authorHugo, Martín-
dc.identifier.citationCásedas, G.; Les, F.; Choya-Foces, C.; Hugo, M.; López, V. The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent. Antioxidants 2020, 9, 177.es_ES
dc.descriptionUrolithin A is a metabolite generated from ellagic acid and ellagitannins by the intestinal microbiota after consumption of fruits such as pomegranates or strawberries. The objective of this study was to determine the cytoprotective capacity of this polyphenol in Neuro-2a cells subjected to oxidative stress, as well as its direct radical scavenging activity and properties as an inhibitor of oxidases. Cells treated with this compound and H2O2 showed a greater response to oxidative stress than cells only treated with H2O2, as mitochondrial activity (MTT assay), redox state (ROS formation, lipid peroxidation), and the activity of antioxidant enzymes (CAT: catalase, SOD: superoxide dismutase, GR: glutathione reductase, GPx: glutathione peroxidase) were significantly ameliorated; additionally, urolithin A enhanced the expression of cytoprotective peroxiredoxins 1 and 3. Urolithin A also acted as a direct radical scavenger, showing values of 13.2 µM Trolox Equivalents for Oxygen Radical Absorbance Capacity (ORAC) and 5.01 µM and 152.66 µM IC50 values for superoxide and 2,2-diphenyss1-picrylhydrazyl (DPPH) radicals, respectively. Finally, inhibition of oxidizing enzymes, such as monoamine oxidase A and tyrosinase, was also detected in a dose-dependent manner. The cytoprotective effects of urolithin A could be attributed to the improvement of the cellular antioxidant battery, but also to its role as a direct radical scavenger and enzyme inhibitor of oxidases.es_ES
dc.format.extent16 p.es_ES
dc.publisherMDPI AGes_ES
dc.relationThis work has been partially financed by Universidad San Jorge and by Spanish Government grants (RTI 2018-094203-B-I00 to A.M.R and IJCI-2017-34170 to M.H.).es_ES
dc.rightsAtribución 4.0 Internacional*
dc.subjectUrolitina Aes_ES
dc.subjectÁcido elágicoes_ES
dc.subjectPolifenoles dietéticoses_ES
dc.titleThe metabolite urolithin-a ameliorates oxidative stress in neuro-2a cells, becoming a potential neuroprotective agentes_ES
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