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dc.contributor.authorHernaiz, Adelaida-
dc.contributor.authorSanz, Arianne-
dc.contributor.authorSentre, Sara-
dc.contributor.authorRanera, Beatriz-
dc.contributor.authorLópez-Pérez, Óscar-
dc.contributor.authorZaragoza, Pilar-
dc.contributor.authorBadiola, Juan J.-
dc.contributor.authorFilali, Hicham-
dc.contributor.authorBolea, Rosa-
dc.contributor.authorToivonen, Janne Markus-
dc.contributor.authorMartin-Burriel, Inmaculada-
dc.date.accessioned2022-04-26T11:52:03Z-
dc.date.available2022-04-26T11:52:03Z-
dc.date.issued2022-02-16-
dc.identifier.citationHernaiz A, Sanz A, Sentre S, Ranera B, Lopez-Pérez O, Zaragoza P, Badiola JJ, Filali H, Bolea R, Toivonen JM and Martín-Burriel I (2022) Genome-Wide Methylation Profiling in the Thalamus of Scrapie Sheep. Front. Vet. Sci. 9:824677. doi: 10.3389/fvets.2022.824677es_ES
dc.identifier.issn2297-1769es_ES
dc.identifier.urihttps://repositorio.usj.es/handle/123456789/772-
dc.description.abstractScrapie is a neurodegenerative disorder belonging to the group of transmissible spongiform encephalopathy (TSE). Scrapie occurs in sheep and goats, which are considered good natural animal models of these TSE. Changes in DNA methylation occur in the central nervous system (CNS) of patients suffering from prion-like neurodegenerative diseases, such as Alzheimer's disease. Nevertheless, potential DNA methylation alterations have not yet been investigated in the CNS of any prion disease model or naturally infected cases, neither in humans nor in animals. Genome-wide DNA methylation patterns were studied in the thalamus obtained from sheep naturally infected with scrapie at a clinical stage (n = 4) and from controls (n = 4) by performing a whole-genome bisulfite sequencing (WGBS) analysis. Ewes carried the scrapie-susceptible ARQ/ARQ PRNP genotype and were sacrificed at a similar age (4-6 years). Although the average genomic methylation levels were similar between the control and the scrapie animals, we identified 8,907 significant differentially methylated regions (DMRs) and 39 promoters (DMPs). Gene Ontology analysis revealed that hypomethylated DMRs were enriched in genes involved in transmembrane transport and cell adhesion, whereas hypermethylated DMRs were related to intracellular signal transduction genes. Moreover, genes highly expressed in specific types of CNS cells and those previously described to be differentially expressed in scrapie brains contained DMRs. Finally, a quantitative PCR (qPCR) validation indicated differences in the expression of five genes (PCDH19, SNCG, WDR45B, PEX1, and CABIN1) that matched the methylation changes observed in the genomic study. Altogether, these results suggest a potential regulatory role of DNA methylation in prion neuropathology.es_ES
dc.format.extent16 p.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherFRONTIERS MEDIA SAAVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE CH-1015, SWITZERLANDes_ES
dc.relationHernaiz A, Sanz A, Sentre S, Ranera B, Lopez-Pérez O, Zaragoza P, Badiola JJ, Filali H, Bolea R, Toivonen JM and Martín-Burriel I (2022) Genome-Wide Methylation Profiling in the Thalamus of Scrapie Sheep. Front. Vet. Sci. 9:824677. doi: 10.3389/fvets.2022.824677es_ES
dc.relation.requiresAdobe pdfes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDNA methylationes_ES
dc.subjectThalamuses_ES
dc.subjectOvine scrapiees_ES
dc.subjectPriones_ES
dc.subjectWhole genome bisulfite sequencinges_ES
dc.titleGenome-Wide Methylation Profiling in the Thalamus of Scrapie Sheepes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fvets.2022.824677/fulles_ES
dc.identifier.publicationfirstpage1es_ES
dc.identifier.publicationlastpage16es_ES
dc.identifier.doi10.3389/fvets.2022.824677es_ES
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses_ES
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